Identification and characterization of nematode specific protective epitopes of Brugia malayi TRX towards development of synthetic vaccine construct for lymphatic filariasis.
Identifieur interne : 005856 ( Main/Exploration ); précédent : 005855; suivant : 005857Identification and characterization of nematode specific protective epitopes of Brugia malayi TRX towards development of synthetic vaccine construct for lymphatic filariasis.
Auteurs : Jayaprakasam Madhumathi [Inde] ; Prabhu Rajaiah Prince ; Gandhirajan Anugraha ; Pote Kiran ; Donthamsetty Nageswara Rao ; Maryada Venkata Rami Reddy ; Perumal KalirajSource :
- Vaccine [ 1873-2518 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Brugia malayi (immunologie), Cytokines (immunologie), Déterminants antigéniques des lymphocytes B (immunologie), Déterminants antigéniques des lymphocytes T (immunologie), Femelle, Filariose lymphatique (), Filariose lymphatique (immunologie), Humains, Immunoglobuline G (sang), Lymphocytes T (immunologie), Murinae, Mâle, Prolifération cellulaire, Souris, Souris de lignée BALB C, Thiorédoxines (immunologie), Vaccins conjugués (immunologie), Épitopes immunodominants (immunologie).
- MESH :
- immunologie : Brugia malayi, Cytokines, Déterminants antigéniques des lymphocytes B, Déterminants antigéniques des lymphocytes T, Filariose lymphatique, Lymphocytes T, Thiorédoxines, Vaccins conjugués, Épitopes immunodominants.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- Animaux, Femelle, Filariose lymphatique, Humains, Murinae, Mâle, Prolifération cellulaire, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Brugia malayi (immunology), Cell Proliferation, Cytokines (immunology), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Epitopes, B-Lymphocyte (immunology), Epitopes, T-Lymphocyte (immunology), Female, Humans, Immunodominant Epitopes (immunology), Immunoglobulin G (blood), Male, Mice, Mice, Inbred BALB C, Murinae, T-Lymphocytes (immunology), Thioredoxins (immunology), Vaccines, Conjugate (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- immunology : Brugia malayi, Cytokines, Elephantiasis, Filarial, Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte, Immunodominant Epitopes, T-Lymphocytes, Thioredoxins, Vaccines, Conjugate.
- prevention & control : Elephantiasis, Filarial.
- Animals, Cell Proliferation, Female, Humans, Male, Mice, Mice, Inbred BALB C, Murinae.
Abstract
Although multi-epitope vaccines have been evaluated for various diseases, they have not yet been investigated for lymphatic filariasis. Here, we report for the first time identification of two immunodominant B epitopes (TRXP1 and TRXP2) from the antioxidant Brugia malayi thioredoxin by studying their immune responses in mice model and human subjects. TRXP1 was also found to harbor a T epitope recognized by human PBMCs and mice splenocytes. Further, the epitopic peptides were synthesized as a single peptide conjugate (PC1) and their prophylactic efficacy was tested in a murine model of filariasis with L3 larvae. PC1 conferred a significantly high protection (75.14%) (P < 0.0001) compared to control (3.7%) and recombinant TRX (63.03%) (P < 0.018) in experimental filariasis. Our results suggest that multi-epitope vaccines could be a promising strategy in the control of lymphatic filariasis.
DOI: 10.1016/j.vaccine.2010.05.012
PubMed: 20653106
Affiliations:
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Pote" uniqKey="Kiran P" first="Pote" last="Kiran">Pote Kiran</name></author><author><name sortKey="Rao, Donthamsetty Nageswara" sort="Rao, Donthamsetty Nageswara" uniqKey="Rao D" first="Donthamsetty Nageswara" last="Rao">Donthamsetty Nageswara Rao</name></author><author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></analytic><series><title level="j">Vaccine</title><idno type="eISSN">1873-2518</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Brugia malayi (immunology)</term><term>Cell 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lymphatique (immunologie)</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Lymphocytes T (immunologie)</term><term>Murinae</term><term>Mâle</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Thiorédoxines (immunologie)</term><term>Vaccins conjugués (immunologie)</term><term>Épitopes immunodominants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Brugia malayi</term><term>Cytokines</term><term>Déterminants antigéniques des lymphocytes B</term><term>Déterminants antigéniques des lymphocytes T</term><term>Filariose lymphatique</term><term>Lymphocytes T</term><term>Thiorédoxines</term><term>Vaccins conjugués</term><term>Épitopes immunodominants</term></keywords><keywords scheme="MESH" qualifier="immunology" 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Here, we report for the first time identification of two immunodominant B epitopes (TRXP1 and TRXP2) from the antioxidant Brugia malayi thioredoxin by studying their immune responses in mice model and human subjects. TRXP1 was also found to harbor a T epitope recognized by human PBMCs and mice splenocytes. Further, the epitopic peptides were synthesized as a single peptide conjugate (PC1) and their prophylactic efficacy was tested in a murine model of filariasis with L3 larvae. PC1 conferred a significantly high protection (75.14%) (P < 0.0001) compared to control (3.7%) and recombinant TRX (63.03%) (P < 0.018) in experimental filariasis. Our results suggest that multi-epitope vaccines could be a promising strategy in the control of lymphatic filariasis.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Anugraha, Gandhirajan" sort="Anugraha, Gandhirajan" uniqKey="Anugraha G" first="Gandhirajan" last="Anugraha">Gandhirajan Anugraha</name><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Kiran, Pote" sort="Kiran, Pote" uniqKey="Kiran P" first="Pote" last="Kiran">Pote Kiran</name><name sortKey="Prince, Prabhu Rajaiah" sort="Prince, Prabhu Rajaiah" uniqKey="Prince P" first="Prabhu Rajaiah" last="Prince">Prabhu Rajaiah Prince</name><name sortKey="Rao, Donthamsetty Nageswara" sort="Rao, Donthamsetty Nageswara" uniqKey="Rao D" first="Donthamsetty Nageswara" last="Rao">Donthamsetty Nageswara Rao</name><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></noCountry><country name="Inde"><noRegion><name sortKey="Madhumathi, Jayaprakasam" sort="Madhumathi, Jayaprakasam" uniqKey="Madhumathi J" first="Jayaprakasam" last="Madhumathi">Jayaprakasam Madhumathi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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